GUT BARRIER INTEGRITY HOW INTESTINAL PERMEABILITY DRIVES SYSTEMIC INFLAMMATION HEALTHY BARRIER GUT LUMEN food bact. fibre IgA TJ TJ TJ TJ TJ BLOODSTREAM Nutrients absorbed · pathogens blocked ✓ Secretory IgA protective Normal sIgA: 510–2040 mg/g on GI-MAP COMPROMISED BARRIER GUT LUMEN LPS toxin pathogen gluten candida ZONULIN ↑ BLOODSTREAM — IMMUNE ALERT IgG IL-6↑ TNF↑ Undigested proteins · toxins · pathogens entering ↑ Calprotectin · ↑ Zonulin on GI-MAP Skin: eczema, acne, psoriasis Brain: fog, anxiety, mood · Joints: pain Autoimmune trigger: molecular mimicry MEASURED BY GI-MAP sIgA · Zonulin Calprotectin THE 5R GUT RESTORATION PROTOCOL — IN SEQUENCE REMOVE STEP 1 Pathogens Reactive foods Unnecessary meds Alcohol · stress GI-MAP identifies targets precisely REPLACE STEP 2 HCl if low Digestive enzymes Bile support Ox bile if needed Elastase <200 on GI-MAP = deficient REINOCULATE STEP 3 Targeted probiotics Fermented foods Prebiotic fibre Akkermansia support Only after removal — not into infection REPAIR STEP 4 L-glutamine Zinc carnosine Collagen peptides Omega-3 EPA/DHA Heal tight junctions reduce calprotectin REBALANCE STEP 5 · ONGOING Stress management Sleep quality Slow eating Parasympathetic dining The lifestyle that created it must change DETECTIVE HEALTH · STEPHEN DUNCAN MSC FDN-P · GI-MAP TESTING · DETECTIVE-HEALTH.COM